The association between cagL and cagA, vacAs-m, babA genes in patients with gastric cancer, duodenal ulcer, and non-ulcer dyspepsia related to Helicobacter pylori
| Journal | Volume 83 - 2020 |
| Issue | Fasc.3 - Original articles |
| Author(s) | S. Demiryas 1, R. Caliskan 2, S. Saribas 2, S. Akkus 2, N. Gareayaghi 3, S. Kirmusaoglu 4, N.. Kepil 5, H. Dinc 2, H. Dag 6, E. Dagdeviren 6, H.B. Tokman 2, F. Kalayci 7, M. Demirci 8, I. Tasci 1, Y. Erzin 9, K. Bal 9, B. Kocazeybek 2 |
| Full article |
 PAGES 385-392 VIEW FREE PDF |
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Affiliations: (1) Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Genaral Surgery, Istanbul, Turkey
(2) Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey (3) Istanbul Sisli Hamidiye Etfal Training and Research Hospital, Blood Center, University of Health Sciences, Istanbul, Turkey (4) T.C. Haliç University, Faculty of Arts & Sciences, Department of Molecular Biology and Genetics, Istanbul, Turkey (5) Istanbul University- Cerrahpasa, Cerrahpasa Medical Faculty, Department of Pathology, Istanbul, Turkey (6) Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Istanbul, Turkey (7) Istanbul Yeni Yuzyil University, Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey (8) Beykent University Medical Faculty, Department of Medical Microbiology, Istanbul, Turkey (9) Istanbul University-Cerrahpasa, Cerrahpasa Medical Faculty, Department of Gastroenterelogy, Istanbul, Turkey. |
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Introduction: As a component of the cag T4SS, the cagL gene is involved in the translocation of CagA into host cells and is essential for the formation of cag PAI-associated pili between H. pylori and gastric epithelial cells. Aim: We aimed to investigate the clinical association of the cagL gene with other virulence factors (VacA, CagA, EPIYA-C, and BabA protein) of H. pylori strains isolated from GC, duodenal ulcer (DU), and non-ulcer dyspepsia (NUD) cases. Methods: The patient group (PG), including 47 patients (22 GC and 25 DU) and a 25 control group (CG= NUD) were included. Amplification of the H. pylori cagL, cagA, vacA, and babA2 genes and typing of EPIYA motifs were performed by PCR methods. Results: Sixty-one (84.7%) H. pylori strains were detected with cagL (93.6% in SG, 68% in CG). We detected a significant difference between SG and CG for the presence of cagL (p=0.012) but no statistical comparison was done for (≥2) EPIYA-C repeats In the comparison of H. pylori strains with cagA/vacAs1m1 and cagA/ vacAs1m2 and babA2 for the presence of cagL, we could not detect a significant difference (p=1). Conclusion: We detected a significant difference between groups for the presence of cagL genotype (p=0.012). The vacAs1m1 (OR: 2.829), genotypes increased the GC and DU risk by 2.8 times, while multiple (≥2) EPIYA-C repeats incresed the GC and DU risk by 3.524 times. Gender (to be female) (OR: 0.454) decreased the GC and DU risk by inversly decreased in the multivariate analysis Keywords: Helicobacter pylori, cytotoxin-associated gene A (cagA), cytotoxin-associated gene L (cagL), vacuolating cytotoxin A (vacA). |
| The authors declare that they have no conflict of interest. |
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© Acta Gastro-Enterologica Belgica. PMID 33094584 |
